The Catalogue of Cyclopoid copepods ( Crustacea : Copepoda : Cyclopidae ) from Andriana

The catalogue of cyclopoid copepods collected by A. Damian-Georgescu in Romania and deposited in “Grigore Antipa” National Museum of Natural History is presented. It includes 39 species and subspecies belonging to family Cyclopidae (Crustacea: Copepoda). Type material of Eucyclops graeteri intermedius Damian, 1955 is designated. For each species the following information is presented: collecting sites, data on general distribution in Romania and worldwide, and types of inhabited biotopes. Species names are listed in systematical order with indication of current taxonomic status.


Introduction
Cyclopidae is one of the largest crustacean families, including over 1010 species and subspecies (Papa and Hołyńska 2013).It's a widespread group of copepods which invade a variety of aquatic environments and microhabitats.Cyclopoids are generally associated with substrates and are common in littoral and benthic habi-tats, although some species are planktonic and may contribute substantially to zooplankton biomass (Reid and Williamson 2009).Cyclopoid copepods are also very common in groundwater environment (Monchenko 2003, Kováč et al. 2014).
Despite a high ecological importance of the family, the biodiversity of cyclopoids has been still weakly studied in some areas.It is especially true of rarely encountered species inhabiting special biotopes, such as groundwaters (Pospisil 1994, Stoch et al. 2011, Brancelj et al. 2016).
The study of cyclopoids was started in Romania in the 2 nd part of the 19 th century.A series of papers that includes some data on cyclopoids from aquatic reservoirs of Transylvania were published in this period (Daday 1882, 1883, 1897, Gelei 1909).Zooplankton of various reservoirs in the lower Danube area was studied by H. Spandl (1926), who recorded 8 species of cyclopoids in the region.
The study of subterranean Romanian fauna was initiated by P. A. Chappuis.He carried out intensive investigation between 1924-1949 and collected extensive data about copepods from underground aquatic biotopes.On the base of these investigations, Chappuis described several new species of cyclopoids for Romanian fauna (Chappuis 1923(Chappuis , 1925(Chappuis , 1928)).
Data dealing with groundwater cylopoids are presented in an article of Damian (1955), who described a new subspecies Eucyclops graeteri intermedius (Damian, 1955) (syn.Eucyclops macrurus intermedius Damian, 1955) from the springs in the river basin of Arieş.Petkovski (1972) published a list of copepods of the Banat caves.
The most complete data about cyclopoid fauna of Romania have been presented in A. Damian-Georgescu's monograph (1963).In this book, 53 species and subspecies of cyclopoids were recorded for the Romanian fauna, and two of them, Acanthocyclops phreaticus (Chappuis, 1928) and Eucyclops graeteri intermedius, were found only in Romania.The later species is endemic for the Apuseni and Banat Mountains.
Since the cited monograph of the Romanian cyclopoid fauna, only a few investigations on this group have being conducted (Iepure andOarga 2011, Iepure et al. 2016).Some data on the biodiversity of cyclopoid copepods in Romania are contained in hydrobiological investigations which were developed in studies related to the assessment of aquatic habitats (Onciu and Radu 2006, Avram et al. 2009, Meleg et al. 2011, Battes and Măluţan 2012, Battes et al. 2014, Ciorca et al. 2017).
The main goal of the present paper is the revision of copepods of the family Cyclopidae in the Damian-Georgescu collection deposited in the "Grigore Antipa" National Museum of Natural History, Bucharest.

Material and Methods
In 2017, a visit to "Grigore Antipa" National Museum of Natural History (Bucharest) was conducted in the frame of Academic Exchange Programme between Romanian Academy of Science and the National Academy of Science of Ukraine.
The 545 microscopic slides including 330 slides (342 specimens) of cyclopoids were investigated using two microscopes Olympus CX21 and Olympus BX41, and a stereomicroscope Zeiss STEMI 2000.
All the collecting sites were checked; most of them were represented by Romanian fresh waters, except two localities: 1) Împutita (old name), now Vladychen, Bolhrad district, Odessa Region, Ukraine; 2) Srebarna, Nature Reserve in Bulgaria.
Taxonomical position of all species and genera of the family Cyclopidae is according to Dussart and Defaye (2006).

Results
The taxonomy of the cyclopoid species from A. Damian-Georgescu collection was revised.The collection of Copepoda collected by herself, mainly in Romania, consists of 545 microscopic slides; among them, 330 slides belong to 39 species and subspecies, 13 genera and 3 subfamilies of the family Cyclopidae.Within the 53 species and subspecies of Cyclopidae included in Damian-Georgescu's monograph (1963), the Museum Collection contains only 39 species and subspecies.Based on the revision of the collection, a catalogue of the Cyclopidae collected by A. Damian-Georgescu was elaborated.
The given catalogue includes 39 species and subspecies of Cyclopidae and indicates collecting sites, data on distribution in Romania and worldwide, and types of inhabited biotopes.Species names are listed in systematical order with indication of current taxonomic status.
Studied micro-slides have identification label and geographical label, but with no datum of sampling and we can suggest that material was collected between 1955-1975(Damian 1955, Damian-Georgescu 1963, 1975).
Remarks.According to A. Damian-Georgescu the main diagnostic characters of E. graeteri intermedius are following: antennula 12-segmented without long dence hairs on IV segment; posterior lateral margin of fifth thoracic segment with dence long hairs; caudal rami 3.6-4.6times as long as wide and lateral margin of caudal rami with short "serra" (longitudinal row of spinules) comprising 6-7 spinules; third endopodal segment of P4 (P4 enp 3) 1.94 times as long as wide; medial apical spines of P4 enp 3 are twice as long as lateral spine and longer than segment (Damian 1955).This species is similar to E. graeteri graeteri.The two species can be distinguished by 1) the length of antennula: antennula reaching the posterior margin of cephalothorax in E. graeteri intermedius and only the first thoracic segment in E. graeteri graeteri; 2) pediger V is laterally pilose in E. graeteri intermedius, while pediger V without hair laterally in E. graeteri graeteri; 3) length-width proportions of the caudal rami (3.6-4.6:1 in E. graeteri intermedius and 4-5:1 in E. graeteri graeteri); 4) in lateral surface ornamentation of caudal rami in female: a short "serra" is present in E. graeteri intermedius, while E. graeteri graeteri has naked caudal rami; 5) length-width proportions of P4 enp3 (1.94:1 in E. graeteri intermedius and 1.5:1 in E. graeteri graeteri).
Biotope: small water bodies with macrophytes, littoral zone of ponds and lakes (Gaviria 1998).
Examined material: 1 ♀ without information about locality.
have been recorded only on the territory of Romania and could be regarded as rare for Europe.Other studied species have wider distribution and have been recorded in Romania and other European countries.The type material of Eucyclops graeteri intermedius (Damian, 1955) (syn.Eucyclops macrurus intermedius Damian, 1955) is designated in this paper and could be used for the future possible taxonomic revision of Eucyclops graeteri-complex.Revised data on the distribution of 39 species of the family Cyclopidae could be used for ecological monitoring of the biodiversity of cyclopoid copepods in different aquatic reservoirs of Romania and other European countries.Chappuis A (1923) Nouveaux Copépodes cavernicoles des genres Cyclops et Canthocamptus [New cavedwelling copepods of genera Cyclops and Canthocamptus].Bulletin de Societatii de Stiinte din Cluj 1(4): 584-590.[in French] Chappuis PA (1925).Sur les Copépodes et les Syncarides des eaux souterraines de Cluj et des Monts Bihar [On the copepods and syncarids of subterranean waterbodies in Cluj